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Additional resources for Diagnostic Bacteriology Protocols 2nd Edition (Methods in Molecular Biology Vol 345)
2004) Identification and interrogation of highly informative single nucleotide polymorphism sets defined by bacterial multilocus sequence typing databases. J. Med. Microbiol. 53, 35–45. 46. , et al. (2004) Structure of Pseudomonas aeruginosa populations analyzed by single nucleotide polymorphism and pulsed-field gel electrophoresis genotyping. J. Clin. Microbiol. 186, 4228–4237. 47. Spratt, B. G. , and Johnson, A. ), Humana, Totowa, NJ, pp. 323–352. 48. Fredricks, D. N. and Relman, D. A. (1996) Sequence-based identification of microbial pathogens: a reconsideration of Koch’s Postulates.
Starting the Reaction 1. 1. and mix. On-Chip PCR 45 2. Add a 13-µL aliquot to separate tubes containing the DNA template (see Note 11) and mix carefully using the micropipet. 3. Pipet the master mix onto the slide, directly down onto the oligonucleotide array. 4. Immediately seal the reaction droplet by mounting a cover slip (see Note 12) using plastic forceps. Take care not to trap air bubbles when sealing the reaction droplet underneath the cover slip. Always use gloves when handling slides and do not touch the slide on the upper, flat surface containing the spots.
Yuan, M. A. Christel, F. A. Northrup. (2001) A battery-powered notebook thermal cycler for rapid multiplex real-time PCR analysis. Anal. Chem. 73, 286–289. 48. Rudi, K. (2001) Direct DNA based campylobacter diagnostics: rapid methods enable new logistics in the production of Campylobacter free poultry. New Food 4, 25–29. 49. Bruin, G. J. (2000) Recent developments in electrokinetically driven analysis on microfabricated devices. Electrophoresis 21, 3931–3951. DNA Purification for Diagnostics 35 50.