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By Douglas J. Taatjes, Jürgen Roth

Electronic pictures are facts : and may be taken care of as such / Douglas W. Cromey --
Epi-fluorescence microscopy / Donna J. Webb and Claire M. Brown --
Live-cell migration and adhesion turnover assays / J. Lacoste, ok. younger, and Claire M. Brown --
Multifluorescence confocal microscopy : program for a quantitative research of hemostatic proteins in human venous valves / Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill --
Colocalization research in fluorescence microscopy / Jeremy Adler and Ingela Parmryd --
A time-lapse imaging assay to check nuclear envelope breakdown / Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman --
Light sheet microsocopy in mobile biology / Raju Tomer, Khaled Khairy, and Philipp J. Keller --
Image-based high-throughput screening for inhibitors of angiogenesis / Lasse Evensen, Wolfgang hyperlink, and James B. Lorens --
Intravital microscopy to photo membrane trafficking in reside rats / Andrius Masedunskas [and others] --
Imaging non-fluorescent nanoparticles in dwelling cells with wavelength-dependent differential interference distinction microscopy and planar illumination microscopy / Wei sunlight, Lehui Xiao, and Ning Fang --
Laser scanning cytometry : ideas and applications-an replace / Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz --
Laser catch microdissection for protein and NanoString RNA research / Yelena Golubeva [and others] --
Viewing dynamic interactons of proteins and a version lipid membrane with atomic strength microscopy / Anthony S. Quinn [and others] --
Mica functionaliztion for imaging of DNA and protein-DNA complexes with atomic strength microscopy / Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri Lyubchenko --
Measuring the elastic homes of dwelling cells with atomic strength microscopy indentation / Joanna L. MacKay and Sanjay Kumar --
Atomic strength microscopy sensible imaging on vascular endothelial cells / Lilia A. Chtcheglova and Peter Hinterdorfer --
Porosome : the secretory nanomachine in cells / Bhanu P. Jena --
Stereology and morphometry of lung tissue / Christian Mühlfeld, Lars Knudsen, and Matthias Ochs --
A novel mixed imaging/morphometrical strategy for the research of human sural nerve biopsies for medical analysis / Michele A. vonTurkovich [and others] --
Correlative light-electron microscopy as a device to review in vivo dynamics and ultrastructure of intracellular constructions / Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini --
Photooxidation expertise for correlative mild and electron microscopy / Claudia Meisslitzer-Ruppitsch [and others] --
Electron microscopy of endocytic pathways / Carmen Ranftler [and others] --
Morphological research of autophagy / Keisuke Tabata [and others] --
Cytochemical detection of peroxisomes and mitochondria / Nina A. Bonekamp [and others] --
Histochemical detection of lipid droplets in cultured cells / Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto --
Environmental scanning electron microscopy in phone biology / J.E. McGregor [and others] --
Environmental scanning electron microscopy gold immunolabeling in mobile biology / Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi --
High-pressure freezing for scanning transmission electron tomography research of mobile organelles / Paul Walther, Eberhard Schmid, and Katharina Höhn --
MALDI imaging mass spectrometry for direct tissue research / Stephan Meding and Axel Walch.

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Some shutters are built into the microscope while other systems require external shutters between the lamp and the microscope stand (10) (Fig. 1b). The subsequent component in the optical train is the fluorescence filter cube (Fig. 1a, b). The first filter in the cube is the excitation filter (Fig. 1c). It is typically a band pass filter that selects a specific range of wavelengths of light from the white light source in order to excite the fluorophore of interest. For example, for EGFP excitation a 470/40 blue band pass filter could be used.

If the journal agrees to make this change, your replacement PDF proof will be a larger file size and better quality. 8. Conclusions Once upon a time the darkroom technician acted as a “gate keeper” of image processing technology, but the photographic enlarger has been replaced by Adobe Photoshop® and other commercial image editing software. The tricky darkroom procedures that could be done by a few have now been replaced by digital tricks that can performed by almost anyone. Along the way we seem to have lost the distinction between appropriate and inappropriate image manipulations.

PLoS Med 2:e124 50. Hyde DM, Tyler NK, Plopper CG (2007) Morphometry of the respiratory tract: avoiding the sampling, size, orientation, and reference traps. Toxicol Pathol 35:41–48 51. McNamara G (2006) Crusade for Publishing Better Light Micrographs—Light 1 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. Digital Images Are Data: And Should Be Treated as Such Microscope Publication Guidelines. htm. Accessed 16 Sept 2011 North AJ (2006) Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition.

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