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Download Carbohydrate Metabolism - Part E by Willis A. Wood (Eds.) PDF

By Willis A. Wood (Eds.)

The seriously acclaimed laboratory usual, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. given that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this present day - really an important book for researchers in all fields of existence sciences

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Extra info for Carbohydrate Metabolism - Part E

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The cooperative interactions displayed by this enzyme are completely lost in the presence of a saturating concentration of the positive effectors. The resultant hyperbolic saturation curve for fructose 6-phosphate and ATP is consistent with a "Ping-Pong Bi-Bi" mechanism named by Cleland. 4 Thus, the kinetic properties are readily interpreted in terms of the reaction models of "allosteric Ping-Pong II," which is one the reaction models proposed by Sumi and Ui? The model suggests that the conversion of free enzyme to another stable enzyme form is an essential step forallosteric transition.

9. 4 s. c. Supowit and B. G. Harris, Biochim. Biophys. Acta 422, 48 (1976). [5] G L U C O K I N A S E FROM Streptococcus m u t a n s 25 Both sodium dodecyl sulfate gel electrophoresis and Sephadex G-200 column c h r o m a t o g r a p h y give molecular weights in the range of 97,000100,000. Most m a m m a l i a n hexokinases are m o n o m e r s with molecular weights of 100,000, 5 and it is probable that the ascarid e n z y m e is also a m o n o m e r . 3. 2 m M ) is similar to that of hexokinase from various rat tissues.

O. Kaplan, J. Macromol. Sci. AI0, 15 (1976). -Y. Lee, J. H. Yuan, and E. Goldberg, this series, Vol. 89 [61]. 28 3. 8 31 600 (3000)c , The total activity presented is the sum of four individual experiments, and the final specific activity of Hex A is the average from these experiments. b The value in parentheses refers to the actual specific activity of Hex Cs in the homogenate. e The value in parentheses refers to actual fold of protein purification. a m i d e gel e l e c t r o p h o r e s i s is u s e d to d e t e r m i n e t h e p r o t e i n p u r i t y (10% a c r y l a m i d e gels).

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