By John Villadsen, Jens Nielsen, Gunnar Lidén
Integrating contemporary study at the body structure and modelling of bioreactions and bioreactors, the authors current a entire, unified creation to the rules and practices of the sector. The paintings good points approximately a hundred special layout examples and difficulties, lots of that are compatible for hands-on demonstrations on a private machine or for accelerated learn. The textual content will function a hugely instructive advisor for college kids in bioengineering and biotechnology, in addition to biochemical, chemical, and environmental engineering.
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Extra resources for Bioreaction Engineering Principles
And Bp are square matrices with a dimension of, respectively, N x N and Mx M. 1 this is not generally valid since some substrates are taken up by more than one enzymatic system-but for the present purpose of presenting a framework for setting up stoichiometric equations this lack of total generality does not matter. 1. 3). In E. coli (and in certain other bacteria) two PTSs for glucose have been identified, called respectively n glc and n man . To give a correct description of the glucose uptake it is therefore in principle necessary to include two uptake reactions for glucose.
The ratio of F ADH 2 to NADH may vary with the operating conditions, and the P /0 ratio is therefore not constant. For eucaryotes the situation is even more complex. Here some NADH is formed in the cytoplasm (in the glycolysis) and some NADH is formed in the mitochondria. NADH formed in the cytoplasm can not pass the mitochondrial membrane, and its oxidation to NAD+ is coupled to reduction of FAD to F ADH 2 in the mitochondria. Cytoplasmic NADH therefore enters the respiratory chain at the same position as F ADH 2 , and the theoretical P /0 ratio for oxidation of cytoplasmic NADH is only 2.
The oxidative capacity of the cells is not large enough 10 o\idlA' ;t1llhc" ,,:\ ()I! formed in the TCA cycle. To prevent accumulation of NADH inside thc cd!. "'IIl,' "r lhe PYR is converted to acetate instead of being oxidized all the way to CO:. Whel1 I h,' gluc"o,.. is exhausted, acetate may be metabolized to carbon dioxide by the cd!. hut Ih" pr",'"'' I' 1101 described in the present model. Chapter 2 32 Formation of macromolecules and other biomass components is lumped into a single reaction involving all three substrates and one biomass product, X.