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Download Bioconjugation Protocols by Christof M. Niemeyer PDF

By Christof M. Niemeyer

Specialist laboratorians replace the vintage bioconjugation tools and introduce worthwhile new suggestions that transcend natural biomedical purposes to incorporate components from complicated natural synthesis, molecular biology, and fabrics technological know-how. those conveniently reproducible equipment conceal the training of protein conjugates utilizing covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates utilizing numerous labeling ideas, and methods to semisynthetic conjugates of proteins. extra chapters deal with the biofunctionalization of inorganic surfaces, together with the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput research of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic strength microscopy.

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Liu. 1999. Fabrication and imaging of nanometer-sized protein patterns. Langmuir 15 (25):8580–8583. Wang, X. F. and C. Liu. 2005. Multifunctional probe array for nano patterning and imaging. Nano Letters 5 (10):1867–1872. Wang, X. , K. S. R yu, D. A. B ullen, J. Zou, H. Zha ng, C. A. M irkin, and C. L iu. 2003. S canning probe contact printing. Langmuir 19 (21):8951–8955. Wilson, D. , R . Martin, S. H ong, M. Cr onin-Golomb, C. A. M irkin, and D. L. K aplan. 2001. S urface organization and nanopatterning of collagen by dip-pen nanolithography.

Green color represents staining of actin using Alexa Fluor 488 phalloidin, blue represents Hoechst nuclear staining, and red represents anti-vinculin antibody conjugated with TRITC. (a) Image of a single cell and (b) close up of focal adhesion sites. 1 Cell–substratum adhesion. (a) Fluorescent microscope image of a single bovine capillary endothelial cell cultured on a smo oth glass substrate. Cell was stained for v inculin (green) and F-actin (red). Focal adhesions (green) are located at t he tips of stress fibers (inset image).

A. M irkin. 2005. N anoarrays o f sin gle vir us pa rticles. Angewandte Chemie—International Edition 44 (37):6013–6015. Atomic Force Microscope Lithography on Biomimetic Surfaces 1-15 Vega, R. , C. K. F . Shen, D. Maspoch, J. G. Robac h, R. A. L amb, and C. A. M irkin. 2007. M onitoring single-cell infectivity f rom vir us-particle nanoarrays fabricated by parallel dip-pen nanotithography. Small 3 (9):1482–1485. , S. Xu, N. A. Amro, and G. Y. Liu. 1999. Fabrication and imaging of nanometer-sized protein patterns.

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