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Download Baculovirus and Insect Cell Expression Protocols by David W. Murhammer (auth.), David W. Murhammer (eds.) PDF

By David W. Murhammer (auth.), David W. Murhammer (eds.)

Baculovirus Expression Protocols is an in depth advisor for utilizing the baculovirus expression vector process (BEVS) and/or insect cells to supply recombinant proteins. approaches for the advance and construction of baculovirus pesticides at either laboratory and massive scale are defined within the form of step by step layout that the equipment in Molecular Biology™ sequence helped standardize. This ebook is a one-stop resource for info on baculoviruses, and contains sections on utilizing changed baculoviruses to precise genes in mammalian cells, utilizing Drosophila mobilephone strains, keeping apart new mobile traces, and constructing serum-free medium. particular purposes, together with utilizing baculovirus and bug mobile procedure to check apoptosis, also are mentioned. This up to date and increased variation of Baculovirus Expression Protocols offers an ideal lab handbook for biochemists, molecular biologists, biochemical engineers, and others for utilizing the BEVS or insect cellphone traces to supply recombinant proteins.

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Extra resources for Baculovirus and Insect Cell Expression Protocols

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Jones, I. , and Bishop, D. H. (1993) Analysis of poly(A) 58. signals for use in baculovirus expression vectors. Virology 195, 190–199. 59 Chisholm, G. E. and Henner, D. J. (1988) Multiple early transcripts and splicing 59. of the Autographa californica nuclear polyhedrosis virus IE-1 gene. J. Virol. 62, 3193–3200. 60 Lubbert, H. and Doerfler, W. (1984) Transcription of overlapping sets of RNAs 60. from the genome of Autographa californica nuclear polyhedrosis virus: a novel method for mapping RNAs.

Gen. Virol. 71, 1029–1037. 66 Dickson, J. A. and Friesen, P. D. (1991) Identification of upstream promoter ele66. ments mediating early transcription from the 35,000-molecular-weight protein gene of Autographa californica nuclear polyhedrosis virus. J. Virol. 65, 4006–4016. 67 Guarino, L. A. and Smith, M. (1992) Regulation of delayed-early gene transcrip67. tion by dual TATA boxes. J. Virol. 66, 3733–3739. 68 Carson, D. , Summers, M. , and Guarino, L. A. (1991) Transient expression 68. of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements.

A. (1997) Analysis of p74, a PDV envelope protein of Autographa californica nucleopolyhedrovirus required for occlusion body infectivity in vivo. J. Gen. Virol. 78, 3091–3100. , and L—pez Ferber, M. (2002) Characterization of pif, a gene required for the per os infectivity of Spodoptera littoralis nucleopolyhedrovirus. J. Gen. Virol. 83, 3013–3022. Pijlman, G. , Pruijssers, J. , and Vlak, J. M. (2003) Identification of pif-2, a third conserved baculovirus gene required for per os infection of insects.

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