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Download Advances in Stem Cell Research by Martin J. Pfeiffer, Martin Stehling, Anna Jauch (auth.), PDF

By Martin J. Pfeiffer, Martin Stehling, Anna Jauch (auth.), Hossein Baharvand, Nasser Aghdami (eds.)

Advances in Stem mobilephone Research discusses fresh advances in stem phone technological know-how, together with healing purposes. This quantity covers such issues as biomanufacturing iPS cells for healing functions, recommendations for controlling stem mobile destiny judgements, in addition to present simple examine in such parts as germ line stem cells, genomics and proteomics in stem mobilephone study. it's a valuable ebook for biology and medical scientists, specifically younger investigators and stem telephone biology scholars who're newly coming into the area of stem cells examine. The editors wish that the hot wisdom and examine defined during this e-book can assist give a contribution to new remedies for a large choice of ailments that almost immediately afflict humanity.

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Mayshar Y, Ben-David U, Lavon N, Biancotti JC, Yakir B, Clark AT, Plath K, Lowry WE, Benvenisty N (2010) Identification and classification of chromosomal aberrations in human induced pluripotent stem cells. Cell Stem Cell 7(4):521–531 13. Darr H, Mayshar Y, Benvenisty N (2006) Overexpression of NANOG in human ES cells enables feeder-free growth while inducing primitive ectoderm features. Development 133(6):1193–1201 14. Enver T, Soneji S, Joshi C, Brown J, Iborra F, Orntoft T, Thykjaer T, Maltby E, Smith K, Dawud RA, Jones M, Matin M, Gokhale P, Draper J, Andrews PW (2005) Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells.

Another protocol generated definitive endoderm by treating hPSCs with Activin A and sodium butyrate, then EGF, bFGF, Noggin, nicotinamide, and IGF-II were added at various stages to specify pancreatic endoderm [38]. Small molecules which guide hPSC differentiation toward b-cells have also been identified. The compounds IDE1 and IDE2 have been shown to induce definitive endoderm differentiation via activation of TGFb/Nodal signaling [39]. Stauprimide downregulates c-MYC expression and causes hPSCs to preferentially differentiate to definitive endoderm [40].

2 has also been identified in human ES cells. Amplification of this region has been reported in two independent human ES cell lines, and in one case as a homogeneous staining region (HSR), a cytogenetic representation of genetic amplification that is almost unique to cancer cells. The amplification of this region has also been reported in breast cancer [19] retinoblastoma [20], and frequently in osteosarcoma [21], suggesting this region may contain gene(s) which can impact on cell fate. Aside from the changes on chromosomes 12 and 17, gains of material from chromosomes 1, 20 and X have also been seen with relative regularity.

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